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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important purpose in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but comprehensive nucleotide sequence information and facts is just not however readily available.

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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope regarded through the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide not bind for the get more info epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is precise.

Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells development by S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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